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13. Park J, Ha H, Seo J et al: Mycophenolic acid inhibits platelet-derived growth factor-induced reactive species and mitogen activated protein kinase in rat vascular smooth muscle cells. J Transplant, 2004; 12: 198290 Eguchi S, Dempsey PJ, Frank GD et al: Activation of mitogen activated protein kinases by angiotensin II in vascular smooth muscle cells. J Biol Chem, 2001; 276 11 ; : 795762 15. Sayeed S, Cullen JP, Coppage M et al: Ethanol differentially modulates the expression and activity of cell cycle regulatory proteins in rat aortic smooth muscle cells. Eur J Pharmacol, 2002; 445 3 ; : 16370 16. Hendrickson RJ, Cahill PA, McKillop IH et al: Ethanol inhibits mitogenactivated protein kinase activity and growth of vascular smooth muscle cells in vitro. Euro J Pharm, 1998; 362: 25159 Sachinidis A, Gouni-Berthold I, Seul C et al: Early intracellular signaling pathways of ethanol in vascular smooth muscle cells. Br J Pharcol, 1999; 128 8 ; : 176171 18. York JL, Welte J, Hirsch J: Gender comparison of alcohol exposure on drinking occasions. J Stud Alcohol, 2003; 64 6 ; : 790801 19. Britton A, Marmot M: Different measures of alcohol consumption and risk of coronary heart disease and all-cause mortality: 11-year follow-up of the Whitehall II cohort study. Addiction, 2004; 99 1 ; : 10916 20. Miller PM, Anton RF, Egan BM et al: Excessive alcohol consumption and hypertension: clinical implications of current research. J Clin Hypertens, 2005; 7 6 ; : 34651 21. Ceccanti M, Sasso GF, Nocente R et al: Hypertension in early alcohol withdrawal in chronic alcocoholics. Alcohol Alcohol, 2006, 41 1 ; : 510 22. Izevbigie EB, Gutkind JS, Ray PE: Angiotensin II and basic fibroblast growth factor mitogenic pathways in human fetal mesangial cells. Pediatric Research, 2000; 45 5 ; : 61421 23. Saso K, Higashi K, Nomura T et al: Inhibitory effect of ethanol on hepatocyte growth factor-induced DNA synthesis and Ca2 + mobilization in rat hepatocytes. Alcohol Clin Exp Res, 1996; 20 Suppl.9 ; : 330A334A 24. Chen J, Ishac JN, Dent P et al: Effects of ethanol on mitogen-activated protein kinase and stress-activated protein kinase cascades in normal and regenerating liver. Biochem J, 1998; 334: 66976 Diehl AM, Yang SQ, Cote P et al: Chronic ethanol consumption disturbs G-Protein expression and inhibits cAMP-dependent signaling in regenerating rat liver. Hepatology, 1992; 16 5 ; : 121219 26. Singletary KW, Frey RS, Yan W: Effect of ethanol on proliferation and estrogen receptor-alpha expression in human breast cancer cells. Cancer Lett; 2001; 165 2 ; : 13137.
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Into its only recognizable intermediate, 33102, as shown in Fig. 7A, lane 3. Likewise, in 7B2 KO animals, compared with their WT littermates, similar defects in pro-CART processing were found in hypothalamic extracts Fig. 7B, lane 3 versus 4 ; , indicating that PC2 enzymatic activity, conferred by 7B2, is crucial for generating the two bioactive CART forms as shown in vitro. In contrast, as demonstrated in Fig. 7C, no apparent processing defects were seen in generating the two bioactive forms I and II in PC1 3 KO hypothalamic extracts compared with their control littermates lane 4 versus lanes 5 and 6 ; indicating the high efficiency of PC2 in processing these two forms. Also, in PC1 3 KO animals, significant amounts of long pro-CART accumulated and correspondingly, intermediate CART- 33 102 ; was undetectable Fig. 7C, lane 4 ; implying that PC1 3 is mostly responsible for cleavage at the RQLR site of long proCART as demonstrated before in our transfection studies. Also, in control animals WT and PC1 3 heterozygotes ; , we found considerable levels of intermediate CART- 10 89 ; lanes 5 and 6 ; along with some unprocessed short pro-CART, forming a doublet with the 10 89 intermediate, only in heterozygotes lane 5 ; , reinforcing the idea that PC1 3 is the major enzyme cleaving at the single basic residue, Arg-9 see Fig. 5A ; . Note that we detected intermediate CART- 33102 ; at very low levels, in PC1 3 control animals lanes 5 and 6 ; , although it was not evident in PC2 and 7B2 WT littermates Fig. 7, A and B, lane 4 ; , possibly because of differences among the various KO strains.
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Cooperation Abstract This study aims to find out which medical interventions during pregnancy are legally acceptable, and what legal protection should be offered in this context to the foetus and to the pregnant woman. These questions are answered in the light of the foetus's status and the rights of the pregnant woman. In addition, the legal positions of third parties i.e. husband partner begetter, attending physician s ; , researcher s ; are dealt with. The law in this field is not only a matter of national legislation and jurisprudence, but also of international standards. Therefore, the study will also take into account legal developments at the international level. The medical interventions involved are prenatal diagnosis and screening, intra-uterine treatment of the foetus, medical experimentation on the foetus in vivo, and abortion because of foetal anomalies, including termination of pregnancy in the third trimester of gestation. Keywords pregnancy, medical interventions, health law Funding AMC UvA.
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